Anogeissus leiocarpus:

Abstract

The plant of Anogeissus leiocarpusis widely used for medicinal purposes by Kashere people in Gombe State, Nigeria. To provide scientific barking for its utilization, ten different concentrations of ethanolic stem bark extract were prepared and tested on E. coli, K. pneumoniae, P. aeruginosa and S. typhi ESBLs bacteria, using standard procedure and phytochemical components of the plant extract was also determined using GC-MS. The results obtained indicate an antibacterial activity at varying concentrations w/v and zones of inhibition diameter (ZID) ranging from 15.67 ± 0.57 mm to 17.00 ± 1.00 mm with a significance difference (p < 0.05) across bacteria tested. Minimum inhibitory concentration (MIC) were between 66.67 ± 23.1 mg/L to 16.67 ± 5.77 mg/L. Minimum Bactericidal Con- centration and Minimum Bacteriostatic Concentration were between 266.67 ± 2.38 mg/L to 106.67 ± 46.19 mg/L and 266.67 ± 92.37 mg/L to 80.00 ± 0.00 mg/L respectively. The MBC/MIC ratio was bacteriostatic for E. coli (0:53) and S. typhi (7:1) while bactericidal for K. pneumonia (4:1) P. aeruginosa (3:1). Twenty five compounds were identified with Myristoleic acid; Z-7-Tetradecenal; 4,5-Di- methyl-4-Hexen-3-one; 1,15-Hexadecadiene and Linoleoyl chloride as most abundant. A more in depth assessment of A. leiocarpus should be carried out to isolate bioactive individual compounds responsible for specific antibacterial activity.